RT_ SYBR Green Fluor qPCR Mastermix (8)

QIAGEN's RT² SYBR Green Fluor qPCR Mastermix is a real-time PCR reagent designed for SYBR Green-based detection on instruments that use fluorescein as a reference dye. The formulation includes a real-time PCR buffer, a chemically-modified HotStart DNA Taq polymerase, nucleotides, and SYBR Green dye. The tightly controlled HotStart enzyme helps prevent the amplification of primer–dimers and other nonspecific products, supporting high amplification efficiency, sensitivity, and specificity.

This mastermix is highly suited for use with Bio-Rad models iCycler, iQ5, MyiQ, and MyiQ2. It belongs to the RT² SYBR Green qPCR Mastermix family, intended for use with RT² Profiler, RT² lncRNA, EpiTect ChIP, and EpiTect Methyl PCR Arrays. Post-PCR dissociation (melting) curve analysis is recommended to assess product homogeneity and confirm specificity.

QIAGEN also recommends pairing this mastermix with the RT² First Strand Kit for reverse transcription, and notes that RT² qPCR Primer Assays may be used on any real-time instrument. Instrument-specific protocols are available for selected instruments.