RT_ SYBR Green Fluor qPCR Mastermix 25ml

QIAGEN's RT² SYBR Green Fluor qPCR Mastermix is a real-time PCR reagent designed for SYBR Green-based detection on instrumentation that uses fluorescein as a reference dye, including the Bio-Rad iCycler, iQ5, MyiQ, and MyiQ2. The mastermix is intended for use with RT² Profiler, RT² lncRNA, EpiTect ChIP, and EpiTect Methyl PCR Arrays, and is supplied without a passive reference dye.

The formulation contains real-time PCR buffer, a chemically-modified HotStart DNA Taq polymerase, nucleotides, and SYBR Green dye. The tightly controlled HotStart enzyme is designed to prevent the amplification of primer–dimers and other nonspecific products, supporting high amplification efficiency along with high sensitivity and specificity over a wide dynamic range.

Post-PCR dissociation curve analysis is recommended to assess product homogeneity and confirm reaction specificity, which is important given SYBR Green's lack of sequence specificity. The product is for research use only and should be stored frozen, with repeated freeze–thaw cycles avoided.