RSeT Feeder-Free Medium

This defined, xeno-free culture system from STEMCELL Technologies is engineered for the maintenance and expansion of human pluripotent stem cells, including both embryonic stem cells and induced pluripotent stem cells, in a workflow that eliminates the need for a mouse embryonic fibroblast feeder layer. The RSeT Feeder-Free Medium formulation supports clonal expansion and routine single-cell passaging when paired with a ROCK inhibitor such as Y-27632, while preserving expression of core pluripotency markers including OCT4, SSEA-4, and TRA-1-60 over extended passage.

Researchers typically combine the medium with defined attachment matrices such as Corning Matrigel hESC-qualified coating or recombinant vitronectin to establish a fully defined, feeder-free workflow for cell culture applications. The product ships as a liquid bottle in 100 mL and 500 mL sizes, requiring only the addition of the supplied supplement before first use. For routine handling, working stocks are stored at 2 to 8 degrees C and protected from light; the frozen supplement component should be kept at -20 degrees C until reconstitution.

This research use only reagent is validated for in vitro workflows and supports gene editing, directed differentiation into all three germ lineages, and the establishment of cryopreserved pluripotent cell banks, all without animal-derived components.