Repro.& Maint. of hPSCs Course-UK-Spring

A hands-on laboratory training program delivered in the United Kingdom during the spring term, this course from STEMCELL Technologies covers the full workflow of reprogramming somatic cells to human pluripotent stem cells (hPSCs) and maintaining them in culture.

The 3-day intensive combines lectures, demonstrations, and bench work using defined, feeder-free media systems such as mTeSR Plus and ReproTeSR. Participants learn colony picking, passaging techniques, cryopreservation at -80 °C, and quality assessment methods including karyotyping and flow cytometry-based pluripotency marker analysis. Standard 37 °C incubation conditions are demonstrated alongside feeding schedules for both naive and primed hPSC lines.

The curriculum also addresses common culture problems such as spontaneous differentiation, microbial contamination, and genetic drift. Taught by experienced applications scientists, the program keeps class sizes small to maximize individual bench time.

Attendees receive a certificate of completion and take home standard operating procedures, troubleshooting guides, and media formulation documentation. The training is intended for academic researchers, graduate students, and industry scientists building cell therapy, disease modeling, or developmental biology projects who need reproducible, standardized protocols for hPSC handling under research use only conditions.