Repro & Maint of hPSCs Course

Gain hands-on proficiency in establishing and sustaining human pluripotent stem cell (hPSC) cultures through this intensive training course offered by STEMCELL Technologies. The Repro & Maint of hPSCs program delivers practical instruction across essential workflows including somatic cell reprogramming, colony picking, feeder-free maintenance, enzymatic and mechanical passaging, cryopreservation, and directed differentiation assays. Participants work directly with primary cell reagents and TeSR family media under BSL-2 conditions in vitro, learning to recognize undifferentiated colony morphology and troubleshoot spontaneous differentiation events in cell culture. The curriculum also covers characterization methods such as flow cytometry for pluripotency markers, karyotyping quality checks, and mycoplasma contamination screening, equipping researchers with the decision-making framework needed for downstream assay scale-up. Targeted at graduate students, postdoctoral fellows, and core facility staff, the course combines short lectures with extended wet-lab bench time over several days, typically hosted at STEMCELL Technologies training facilities. Enrollment includes a printed course manual, lab consumables, and a certificate of completion; reagents demonstrated in class are documented for direct ordering so that optimized protocols transfer cleanly back to the participant's home laboratory.