Repro,Maint& Diff hPSCs Course-UK-Winter

This intensive 5-day hands-on training course from STEMCELL Technologies provides comprehensive instruction in human pluripotent stem cell (hPSC) technologies, with a curriculum built around three core modules: reprogramming somatic cells to pluripotency, maintaining undifferentiated hPSC cultures, and directing differentiation toward defined lineages. Participants gain practical laboratory experience with feeder-free and feeder-dependent culture systems, working with reagents such as mTeSR™ Plus and TeSR™-E8™ under BSL-2 in vitro conditions at 37°C in 5% CO₂, and learning colony-passaging, cryopreservation, and quality-control characterization techniques including flow cytometry and immunocytochemistry.

The winter UK session combines lectures, live demonstrations, and bench time in small groups to ensure individualized instruction. Topics include somatic cell reprogramming using episomal and mRNA-based methods, troubleshooting culture contamination and spontaneous differentiation, and directed differentiation protocols for ectoderm, mesoderm, and endoderm lineages. By the end of the program, attendees are equipped to establish, scale, and cryobank hPSC lines for translational research and drug-discovery applications, with take-home protocol binders and a certificate of completion issued by STEMCELL Technologies.