Rat Protein Phosphatase, Mg2+/Mn2+ Dependent 1A ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of rat Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) in tissue homogenates, cell lysates and other biological fluids. The assay is reactive with rat samples and employs a colourimetric readout on a microplate reader.

The kit uses a pre-coated 96-well microplate with an antibody specific for PPM1A. Standards and samples are added to the wells, followed by a Biotinylated Anti-PPM1A Antibody and an HRP-Avidin conjugate. TMB substrate is then added, and the enzymatic reaction is terminated with acidic stop solution; absorbance is read at 450 nm. The stated detection range is 0.312–20 ng/ml with a sensitivity of 0.138 ng/ml, and the total assay time is 4 h 30 m. Component storage is at +4 °C or -20 °C as instructed.

Reported average recovery values across sample matrices include serum (range 83–100%, average 91%), EDTA plasma (90–103%, 96%) and heparin plasma (89–102%, 95%). Synonyms listed for the target include GL, HGL and HLAL; the detection antibody host is rabbit (IgG). The product is supplied by Antibodies.com as a research-use-only reagent.