Annexin V-APC Apoptosis Detection Kit

The Annexin V-APC Apoptosis Detection Kit from Antibodies.com enables researchers to identify early apoptotic cells by flow cytometry. Annexin V is a calcium-dependent phospholipid-binding protein that binds phosphatidylserine (PS) residues externalized on the outer leaflet of the plasma membrane during the early stages of programmed cell death. The APC (allophycocyanin) fluorophore provides far-red fluorescence, with excitation near 650 nm and emission around 660 nm, making it compatible with multicolor panels that include PE, FITC, and viability dyes such as 7-AAD or propidium iodide. The 100T kit supplies sufficient annexin V-APC conjugate and 1X binding buffer for 100 flow cytometry staining reactions. Refrigerate at 4 °C, protect from light, and do not freeze; the conjugate is supplied in a single-use vial that should be kept on ice during staining. For research use only, the kit is suitable for in vitro studies of suspension and adherent mammalian cell lines, including cultured tumour cells, primary lymphocytes, and drug-treated samples.