Membrane and Cytosol Protein Extraction Kit

The Antibodies.com Membrane and Cytosol Protein Extraction Kit fractionates cellular lysates into membrane and cytosolic protein pools for downstream analysis. Researchers apply the prepared fractions to western blot, immunoprecipitation, or enzyme activity assays to study protein localization, signal transduction, and receptor trafficking.

The 100T kit provides reagents for 100 sample preparations, including extraction buffers, a membrane solubilization reagent, and a protease inhibitor cocktail to limit proteolysis during workup. A typical workflow involves resuspending cell pellets in cytosolic buffer, separating the soluble fraction by centrifugation at approximately 10,000 x g, then solubilizing the remaining membrane pellet with the second buffer.

Store buffer components refrigerated upon arrival; aliquot working solutions and avoid repeated freeze-thaw cycles to preserve reagent activity. The protocol accommodates cultured mammalian cells and tissue homogenates, and isolated fractions are compatible with SDS-PAGE and downstream mass spectrometry sample preparation.