qBiomarker SYBR Fluor Mastermix (2)

A 2x concentrated SYBR Green-based real-time PCR master mix engineered by QIAGEN for use with qBiomarker pathway-focused arrays. The formulation combines hot-start DNA polymerase, dNTPs, and a proprietary SYBR Fluor intercalating dye in an optimized single-tube buffer, reducing pipetting steps during plate setup. It supports 384-well plate runs on standard real-time thermal cyclers, delivering consistent Ct values across a broad dynamic range of input template.

Store at -20 °C protected from light; the dye component is photosensitive and activity degrades with repeated freeze-thaw cycles. Each vial covers hundreds of reactions depending on plate format, and the mix pairs directly with qBiomarker 384-well arrays covering curated gene sets in apoptosis, cell signaling, inflammation, and oncology pathways. Researchers typically apply this mix in PCR-based screening of pathway-focused gene panels, then confirm differential expression with individual qPCR assays, Western blot validation, or sequencing.