PneumaCult-Ex Plus Medium

This defined, serum- and BPE-free cell culture medium from STEMCELL Technologies supports the expansion of primary human airway epithelial cells. It enables more expansion at each passage compared to other commercially available expansion media and supports at least two additional passages of cell expansion with better differentiation potential, defined as the ability to form a pseudostratified mucociliary epithelium at the air-liquid interface (ALI) using PneumaCult-ALI Medium.

Together, this medium and the ALI medium constitute a fully integrated BPE-free culture system for in vitro human airway modeling. The system is described as a valuable tool for basic respiratory research, toxicity studies, and drug development.