PancreaCult OGM Mouse Kit

The PancreaCult OGM Mouse Kit from STEMCELL Technologies is a specialized serum-free culture system engineered to establish, sustain, and expand organoids derived from murine pancreatic ductal epithelium. Supporting translational cancer research, this complete workflow enables organoid derivation from both healthy pancreatic tissue and diseased specimens, including KRAS-driven pancreatic ductal adenocarcinoma (PDAC) models. Researchers embed dissociated ductal fragments or sorted epithelial cells in a dome of Corning Matrigel or comparable extracellular matrix, then overlay with the supplied medium components to generate three-dimensional structures that faithfully recapitulate the architecture and genetic profile of the parent tissue.

Each kit includes the OGM basal medium plus a rigorously formulated supplement cocktail containing R-spondin, Noggin, EGF, and additional niche factors required to maintain stem cell populations. The system is validated for long-term passaging, with cultures routinely maintained beyond passage 15 while retaining differentiation capacity. Components are shipped frozen and should be stored at -20°C, with the basal medium stable at 4°C after thawing for short-term use. Applications span drug screening, CRISPR-based gene editing of primary pancreatic cultures, co-culture with stromal fibroblasts, and biobanking efforts aimed at preserving patient-derived and mouse-derived tumor avatars for preclinical evaluation.

This research use only platform is cited extensively in studies of pancreatic cancer initiation, acinar-to-ductal metaplasia, and tumor-stroma crosstalk. STEMCELL Technologies supplies protocol documentation covering tissue digestion, embedding density recommendations, and feeding schedules, along with troubleshooting guidance for common contamination or differentiation issues encountered during primary organoid derivation.