N2 Supplement-B (100X), 5mL

For neural cell culture and neuron differentiation workflows, this serum-free formulation provides a defined mix of essential nutrients that supports survival and growth of post-mitotic neurons, glial precursors, and neural stem cell-derived populations. Supplied as a 100X concentrate in a 5 mL bottle, the supplement is added directly to basal medium at 1% v/v, eliminating the variability introduced by batch-to-batch serum lots. The B formulation is optimized for culturing neural cells in combination with NeuroCult basal media and is compatible with standard incubators operating at 37 °C with 5% CO2. The active components contribute insulin, transferrin, progesterone, putrescine, and selenium in defined ratios, supporting membrane integrity, antioxidant defense, and neurotrophic signaling during long-term differentiation protocols. Store frozen at -20 °C and protect from light; thaw aliquots at room temperature and avoid repeated freeze-thaw cycles to preserve the bioactivity of labile components. Each lot is performance tested in dopaminergic neuron and oligodendrocyte differentiation assays to confirm consistent plating efficiency and process formation. Provided by STEMCELL Technologies as a research use only reagent suitable for in vitro protocols in academic and industrial neuroscience laboratories, this single-use format is intended for routine supplementation of neural maintenance and differentiation media.