Mouse Transforming Growth Factor beta 1 ELISA Kit

Supplied through AFMS, the authorized Saudi distributor of Antibodies.com, this 96-well sandwich ELISA kit is designed for the in vitro quantitative determination of mouse Transforming Growth Factor beta 1 (TGF-β1). Built on Antibodies.com's standard sandwich enzyme-linked immunosorbent assay (sELISA) format, the kit uses a pre-coated 96-well microplate (12 × 8 well strips) to capture TGF-β1 from serum, plasma, cell culture supernatant, cell or tissue lysates, and other biological fluids. Capture of the analyte is followed by addition of a biotinylated detection antibody and HRP-Streptavidin conjugate, with TMB substrate producing a blue product that converts to yellow upon stop solution addition; absorbance is read at 450 nm and concentrations are interpolated from the standard curve. Typical Antibodies.com Mouse TGF-β1 ELISA specifications include a quantitative standard range of approximately 31.25–2000 pg/mL, a sensitivity of ~18.75 pg/mL, colorimetric detection, mouse reactivity, and a no-cross-reactivity profile against related TGF-β analogs. The kit is shipped at +4°C, with components stored at +4°C or -20°C according to the protocol manual, and the assay is intended for research use only (not for diagnostic or therapeutic applications). The catalog size is 96 tests per kit, making it well suited to mid-throughput cytokine quantification in immunology, fibrosis, and inflammation studies.