MethoCult SF M3436

STEMCELL Technologies offers a serum-free, methylcellulose-based medium formulated with recombinant cytokines, including EPO, designed for mouse erythroid progenitor cell assays. The formulation is optimized for the growth and enumeration of primitive erythroid progenitor cells (BFU-E) in colony-forming unit (CFU) assays of mouse bone marrow, fetal liver, spleen, peripheral blood, and purified cells.

It does not support the growth of granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M) or multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). The medium is compatible with STEMvision™ software for automated colony counting of mouse bone marrow CFU assays.

The same formulation can also be applied to the growth and enumeration of BFU-E in CFU assays using rat bone marrow cells.