MethoCult M3231, 80mL

STEMCELL Technologies offers a serum-containing methylcellulose formulation built for hematopoietic colony-forming unit (CFU) assays when researchers want to add their own recombinant cytokine combinations rather than rely on a pre-mixed growth factor cocktail. The viscous matrix supports in vitro clonal growth of erythroid, myeloid, and multipotent progenitors while keeping baseline background signals low, enabling tailored readouts from bone marrow, cord blood, and peripheral blood mononuclear cell populations drawn from both human and murine sources.

The 80 mL bottle supplies sufficient volume for approximately 40 to 80 standard 35 mm culture dishes at 1 to 2 mL per dish, with typical plating densities of 1 x 10^5 to 2 x 10^5 nucleated cells. Storage at -20°C preserves lot-to-lot viscosity and clonogenic performance, and thawed bottles kept refrigerated should be used within a short working window to protect assay reproducibility. Each lot is qualified to deliver consistent BFU-E, CFU-GM, and CFU-GEMM colony morphology across replicate dishes, supporting downstream scoring, imaging, and replating workflows in stem cell research laboratories.