MethoCult H4434 Classic, 3mL

A methylcellulose-based medium from STEMCELL Technologies, MethoCult H4434 Classic is formulated for performing colony-forming unit (CFU) assays with human hematopoietic progenitor cells. The 3 mL vial format provides a ready-to-use matrix that supports the simultaneous growth of erythroid, myeloid, and multi-lineage colonies—including BFU-E, CFU-E, CFU-G, CFU-M, CFU-GM, and CFU-GEMM—from sources such as bone marrow, mobilized peripheral blood, or cord blood mononuclear cells.

The H4434 formulation contains a defined supplement cocktail of recombinant human cytokines: stem cell factor (SCF), interleukin-3 (IL-3), interleukin-6 (IL-6), granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), and erythropoietin (EPO). The inclusion of EPO makes this medium especially useful for assays that resolve both erythroid and myeloid lineages in the same culture.

Each 3 mL vial is sufficient to seed approximately four 6-well plates or eight 12-well plates at 1 mL per well, supporting standard cell culture workflows. Typical applications include enumeration of hematopoietic progenitor cells, characterization of stem and progenitor populations, drug screening for myelotoxic or erythropoietic compounds, and validation of cell therapy products. Cultures are typically scored after 14 days of incubation at 37 °C in a humidified 5% CO₂ atmosphere.