MethoCult GF R3774, 100mL

Colony-forming unit (CFU) assays on human hematopoietic progenitor cells rely on this methylcellulose-based culture medium from STEMCELL Technologies, supplied as a 100 mL bottle in the growth factor-free (GF) format. Researchers add their own recombinant cytokines to tailor erythroid, myeloid, and megakaryocytic readout conditions for bone marrow, peripheral blood, or cord blood-derived samples.

The viscous matrix delivers a working methylcellulose concentration of approximately 2.7%, anchoring developing colonies in situ so they can be scored after 14 days of incubation at 37 °C in a humidified 5% CO2 atmosphere. Because no cytokines are pre-included, the formulation offers flexibility to titrate stem cell factor (SCF), interleukin-3 (IL-3), interleukin-6 (IL-6), granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), and erythropoietin (EPO) at experimentally chosen concentrations.

For routine workflows, 1.1 mL of medium per 35 mm culture dish yields roughly 90 dishes per bottle, supporting CFU-GM, BFU-E, and CFU-GEMM enumeration in basic hematology research, drug screening, and progenitor cell characterization studies. The product is intended for in vitro research use only. Store the bottle at -20 °C protected from light, thaw promptly, aliquot to limit freeze-thaw cycles, and return working aliquots to -20 °C for extended stability.