MethoCult GF M3434, 3mL/tube (24/rack)

The hematopoietic progenitor colony-forming unit (CFU) assay relies on a viscous methylcellulose matrix to support clonal expansion of committed myeloid and erythroid progenitors in semi-solid culture. MethoCult GF M3434 from STEMCELL Technologies is supplied as a complete, pre-mixed formulation containing recombinant stem cell factor (SCF), interleukin-3 (IL-3), interleukin-6 (IL-6), and erythropoietin (EPO), removing the need for users to add exogenous cytokines during plating. The medium is widely used in mouse hematopoiesis research to grow erythroid bursts (BFU-E), granulocyte (CFU-G), macrophage (CFU-M), granulocyte-macrophage (CFU-GM), and multilineage (CFU-GEMM) colonies from bone marrow, spleen, or fetal liver cells.

Each tube delivers 3 mL of ready-to-use medium, with 24 tubes per rack sized for routine plating in 35 mm dishes or six-well plates in a single batch. Colonies are typically counted after 7 to 14 days of incubation at 37 °C in a humidified 5% CO2 atmosphere, providing quantitative readouts of progenitor frequency in wild-type, transgenic, or drug-treated mouse models. Applications include assessing hematopoietic toxicity, screening candidate therapeutics, and characterizing sorted stem and progenitor populations.

Tubes should be stored at -20 °C, kept frozen until use, and thawed once to limit freeze-thaw damage; the rack configuration supports multiple replicate dishes while ensuring consistent lot-to-lot performance for cell culture workflows.