MethoCult GF M3434, 100mL

This methylcellulose-based medium from STEMCELL Technologies supports clonal colony-forming unit (CFU) assays of murine hematopoietic progenitors, providing a viscous semi-solid matrix in which individual lineage-committed and primitive cells proliferate into morphologically distinguishable colonies. The pre-mixed recombinant cytokine blend contains murine SCF, IL-3, and GM-CSF together with human IL-6 and erythropoietin, driving simultaneous outgrowth of BFU-E and CFU-E erythroid bursts, CFU-G and CFU-M granulocyte-macrophage colonies, and primitive CFU-GEMM mixed-lineage colonies from low-density mouse bone marrow or spleen suspensions.

For assay setup, cells are typically plated at 1–5 × 10⁴ per 35 mm dish in approximately 1.1 mL of medium, then incubated at 37 °C in a humidified 5% CO₂ atmosphere for 7 to 14 days before scoring. Colonies are enumerated by morphology under an inverted microscope using standard published criteria; the 100 mL single-use bottle yields roughly 90 replicate dishes, removing the need to prepare individual growth factor cocktails and reducing inter-laboratory variability.

Store frozen at -20 °C and protect from light to preserve recombinant cytokine activity; thaw, aliquot into working volumes, and refreeze to limit repeated freeze-thaw cycles. The product is intended for research use only and supports in vitro applications including hematopoietic stem and progenitor cell enumeration, gene-function studies, radiation biology, and drug-response profiling of murine hematopoietic lineages.