MethoCult GF H84434, 100mL

A methylcellulose-based medium with recombinant cytokines from STEMCELL Technologies, formulated for colony-forming unit (CFU) assays to detect and quantify human hematopoietic progenitor cells in bone marrow, mobilized peripheral blood, peripheral blood, and cord blood samples. It is recommended for CD34+ enriched cells, mononuclear cells, and cells isolated by other purification methods.

The formulation supports optimal growth of erythroid progenitor cells (CFU-E and BFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-M, CFU-G), and multi-potential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). Components include Iscove's MDM, methylcellulose, fetal bovine serum, bovine serum albumin, 2-mercaptoethanol, recombinant human stem cell factor, rh GM-CSF, rh G-CSF, rh interleukin 3, and rh erythropoietin (EPO).

Each batch is sterility tested according to USP methods and quality control performance tested in CFU assays using human bone marrow, cord blood, or peripheral blood samples; a Certificate of Analysis is available on request. The product is for in vitro diagnostic use by trained personnel, contains material of animal origin, and may cause allergic reaction in sensitized individuals. Store at -15 to -25°C and do not repeatedly freeze and thaw. Cultures are incubated at 37°C with 5% CO₂ in air and ≥ 95% humidity for 14–16 days, then counted by inverted microscopy or with the STEMvision™ instrument for automated imaging and counting.