ImmunoCult-SF Macrophage Medium, 250mL

STEMCELL Technologies has developed this serum-free medium for the in vitro culture and differentiation of human monocytes into macrophages when the appropriate cytokines and stimuli are added. The 250 mL formulation contains pre-tested bovine serum albumin, insulin, transferrin, and other supplements in Iscove's MDM, with macrophage differentiation and activation factors deliberately omitted so researchers can customize the medium to their requirements.

When supplemented with Human Recombinant M-CSF to a final concentration of 50 ng/mL, the medium supports differentiation of human monocytes into M1 (classically activated) or M2 (alternatively activated) macrophages over a 6- or 8-day culture period. It is stored at -20°C and remains stable until the expiry date on the label. The product contains material derived from human plasma; donors have been tested and found negative for HIV-1 and -2, hepatitis B, and hepatitis C prior to donation, and it should be considered potentially infectious and handled with universal precautions.