Human Thrombospondin Type I Domain Containing Protein 7A ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com for the in vitro quantitative determination of human Thrombospondin Type I Domain Containing Protein 7A (THSD7A) in tissue homogenates, cell lysates or other biological fluids. The assay employs the sandwich enzyme immunoassay technique, using an antibody specific for THSD7A pre-coated onto a 96-well microplate arranged as 12 x 8 well strips. Captured analyte is detected with a Biotinylated Anti-THSD7A Antibody and HRP-Avidin conjugate, with signal developed using TMB substrate solution and read at 450 nm on a colorimetric microplate reader.

The stated assay range is 0.156-10 ng/ml and sensitivity is <0.058 ng/ml, with a total assay time of 4h 30m. Sample recovery was assessed in serum, EDTA plasma, and heparin plasma (n=5 each), with average recovery values of 91% (range 83%-100%), 96% (range 91%-101%), and 96% (range 91%-101%) respectively. Dilution linearity ranges are also reported for these three matrices at 1:2, 1:4, 1:8, and 1:16 dilutions. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date. THSD7A is also referred to by the synonyms KIAA0960 and Thrombospondin type-1 domain-containing protein 7A.