Human Syntenin 2 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of human Syntenin 2 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Syntenin 2 is pre-coated onto a 96-well microtiter plate. Standards and test samples are added to the wells, where the target is captured by the immobilised antibody. After washing, a Biotinylated Anti-Syntenin 2 Antibody binds the captured Syntenin 2, followed by incubation with HRP-Avidin conjugate. TMB substrate is then added, producing a blue product that turns yellow upon addition of acidic stop solution. Absorbance is measured at 450nm in a microplate reader, and the Syntenin 2 concentration is calculated from a standard curve.

The assay range is 0.156–10 ng/ml with a sensitivity of <0.071 ng/ml, and the total assay time is 4 hours 30 minutes. Detection is colourimetric on a pre-coated microplate platform, read on a colorimetric microplate reader. Kit components should be stored at +4°C or –20°C as instructed, and the kit must not be used past its expiration date. Reported recovery in undiluted samples averages 91% for serum (range 80–102%), 90% for EDTA plasma (range 81–100%), and 84% for heparin plasma (range 80–89%); per-dilution recovery ranges are also reported across 1:2, 1:4, 1:8, and 1:16 dilutions for all three matrices.