Human Poly A Binding Protein Cytoplasmic 1 Like Protein ELISA Kit

From Antibodies.com, this sandwich ELISA is designed for the in vitro quantitative determination of human PABPC1L (Poly A Binding Protein Cytoplasmic 1 Like Protein) in tissue homogenates or other biological fluids. The format uses a pre-coated microplate together with a biotinylated anti-PABPC1L detection antibody and HRP-avidin conjugate, with colour development via TMB substrate. Absorbance is read at 450 nm on a colorimetric microplate reader. The stated assay range is 0.312-20 ng/ml with a sensitivity of <0.115 ng/ml, and the protocol takes 4h 30m to complete.

Kit components include a pre-coated microplate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, stored at +4°C or -20°C as instructed. Reported mean recovery is 91% in serum (range 83-100%), 94% in EDTA plasma (90-98%) and 95% in heparin plasma (89-102%) across n=5 samples per matrix, with dilution linearity demonstrated from 1:2 to 1:16.