Human Lactoperoxidase ELISA Kit

Manufactured by Antibodies.com, this Human Lactoperoxidase (LPO) ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay intended for the in vitro quantitative determination of human LPO in breast milk, tears, or other biological fluids. The assay uses a pre-coated microplate format with colorimetric detection, and absorbance is read at 450 nm in a microplate reader. The total assay time is 4 hours 30 minutes.

Key performance characteristics reported by the manufacturer include an assay range of 0.156-10 ng/ml and a sensitivity of <0.057 ng/ml. The kit is validated for human reactivity. Recovery has been characterised across serum (average 91%, range 80%-102%), EDTA plasma (average 90%, range 81%-100%), and heparin plasma (average 84%, range 80%-89%) samples.

Kit components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers. The kit is also offered through third-party distributors, with validation for ELISA applications noted by independent suppliers.