Human Interleukin-2 Soluble Receptor alpha ELISA Kit

Designed for the in vitro quantitative determination of human Interleukin-2 Soluble Receptor alpha (sIL-2Ra, also known as CD25, IL2-RA, p55 and TAC antigen), this 96-T sandwich ELISA from Antibodies.com delivers a ready-to-run colorimetric workflow on a pre-coated microplate arranged as 12 x 8-well strips. Standards or samples in cell culture supernatant, serum, or plasma are pipetted into the wells and bound by an immobilized monoclonal capture antibody specific for human sIL-2Ra; a biotin-linked monoclonal detection antibody and streptavidin-HRP then complete the sandwich, with a TMB substrate read at 450 nm producing a colorimetric signal proportional to analyte concentration. The kit detects both natural and recombinant human sIL-2Ra with a reported sensitivity of 2.99 pg/mL and a standard curve covering 78.13 - 5000 pg/mL, and shows no significant cross-reactivity with related cytokines or receptors. Recovery from serum samples averages 102%, supporting confident quantitation across the dynamic range. The format, sensitivity and broad sample-type coverage make this Antibodies.com kit well suited to immunological, oncology and transplantation research workflows where reliable measurement of soluble CD25 is required.