Human Integrin Beta 3 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of human Integrin Beta 3 in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

The kit uses a pre-coated microplate arranged as 12 x 8 well strips with a colourmetric readout measured at 450 nm. Standards and samples bind to the immobilised capture antibody; a biotinylated anti-Integrin Beta 3 antibody followed by HRP-avidin conjugate and TMB substrate generate a blue product that turns yellow upon addition of stop solution, with absorbance proportional to Integrin Beta 3 concentration. The product range is 31.2–2000 pg/ml with a sensitivity of <13.3 pg/ml, and total assay time is 4 hours 30 minutes. Kit components include the pre-coated plate, lyophilised standard, detection solutions, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, stored at +4°C or –20°C as instructed.

Recovery was validated in serum (range 83–99%, average 91%), EDTA plasma (range 80–99%, average 89%) and heparin plasma (range 82–99%, average 90%). Dilution linearity was demonstrated from 1:2 to 1:16 across these matrices.