Human Ephrin Type A Receptor 10 ELISA Kit

A sandwich ELISA from Antibodies.com is designed for the in vitro quantitative determination of human Ephrin Type A Receptor 10 (EPHA10) in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate format with a biotinylated anti-EPHA10 antibody, an HRP-avidin conjugate, and TMB substrate, and is read colorimetrically at 450 nm in a microplate reader. The concentration of EPHA10 in each well is then calculated by reference to a standard curve. The stated assay range is 0.312–20 ng/ml with sensitivity of <0.109 ng/ml, and a typical run time of 4h 30m. Storage instructions call for kit components to be held at +4°C or −20°C as indicated, and not used past the stated expiration date.

Average recovery values reported by the manufacturer across matched sample matrices are 91% (serum), 94% (EDTA plasma), and 95% (heparin plasma), with n=5 per matrix. Linearity upon dilution at 1:2, 1:4, 1:8, and 1:16 is also reported for the same three matrices. The kit is intended for use with human samples.