Human Defensin Beta 103A ELISA Kit

Defensin Beta 103A (also called hBD-3) is an epithelial-derived antimicrobial peptide central to innate mucosal immunity, and this quantitative immunoassay from Antibodies.com enables researchers to measure its concentration in research samples with high specificity for the human isoform. The kit uses a paired-antibody configuration built around monoclonal capture and detection reagents directed against the Defensin Beta 103A sequence, with no significant cross-reactivity against closely related beta-defensin family peptides.

Each plate provides 96 individual test positions pre-coated and ready for single-use, and the kit includes a recombinant standard, detection antibody concentrate, wash buffer, and substrate solution sufficient to complete one full run. The protocol is straightforward, completed in roughly four to five hours at room temperature with no specialized instrumentation beyond a standard absorbance microplate reader. Sample volume requirements are modest at 50 uL per well, supporting work with cell culture supernatant, tissue lysate, and other research-derived specimens. Scientists studying host defense, epithelial barrier function, or inflammatory signaling pathways will find this a focused tool for quantifying Defensin Beta 103A as part of broader immunological investigations.