Human AE Binding Protein 1 ELISA Kit

A sandwich ELISA from Antibodies.com for the in vitro quantitative determination of human AEBP1 (AE Binding Protein 1) in tissue homogenates, cell lysates, or other biological fluids. The assay is reactive with human samples.

The kit uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and follows a biotinylated anti-AEBP1 antibody, HRP-Avidin conjugate, and TMB substrate workflow, with absorbance read at 450 nm on a colorimetric microplate reader. Total assay time is 4 hours 30 minutes. The stated assay range is 0.312-20 ng/ml and sensitivity is 0.126 ng/ml.

Reported spike-and-recovery performance across five replicates shows average values of 91% for serum (range 80%-102%), 90% for EDTA plasma (range 81%-100%), and 84% for heparin plasma (range 80%-89%), with linearity-of-dilution from 1:2 to 1:16 yielding recoveries from 74% to 108% across the three matrices. Kit components are stored at +4°C or -20°C as indicated on each vial, and the kit should not be used past its expiration date.