HOT FIREPol® Probe qPCR Mix Plus (ROX)

Manufactured by Solis BioDyne in Estonia, this 5x master mix is engineered for real-time quantitative PCR using sequence-specific fluorogenic DNA-probe chemistries. The formulation supports the amplification of up to two targets in a single reaction and contains all components required for qPCR with the exception of template, primers, and probe.

At its core is HOT FIREPol® DNA Polymerase, supplied in a proprietary reaction buffer that enables detection of low-copy-number targets. The mix is optimized for DNA hydrolysis probes based on 5' flap endonuclease activity. A hot-start activation step of 12 minutes at 95°C prevents extension of non-specifically annealed primers and primer-dimers formed during reaction setup. The 5x Probe qPCR buffer delivers a final MgCl2 concentration of 3 mM at 1x, and dNTPs are included alongside an internal reference dye based on ROX, which normalizes fluorescent reporter signals and is compatible with both low- and high-ROX instruments.

Typical applications include detection and quantification of DNA and cDNA targets, gene expression profiling, microbial detection, and viral load determination. The manufacturer recommends reaction setup at room temperature, ideally under laminar flow or in a PCR cabinet. Routine storage is at -18°C to -28°C, and shipping or short-term storage at room temperature for up to one month has no detrimental effect on product quality. The product is manufactured under ISO 9001 and ISO 13485 certified quality management and is supplied for research use only, not for use in diagnostic procedures.