HOT FIREPol® Probe qPCR Mix Plus (Capillary)

Optimized for real-time quantitative PCR, this 5x probe-based master mix from Solis BioDyne contains all components required to perform qPCR except template, primers, and probe. It includes HOT FIREPol® DNA Polymerase in a proprietary reaction buffer designed to enable detection of low copy number targets, together with 5x Probe qPCR buffer providing 15 mM MgCl2 (yielding 3 mM MgCl2 in a 1x PCR solution), dNTPs, and BSA. The formulation is optimized for DNA hydrolysis probes based on 5' flap endonuclease activity. The polymerase is activated by a 12 min incubation step at 95ºC, which prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Typical applications include detection and quantification of DNA and cDNA targets, profiling gene expression, microbial detection, and viral load determination. A recommended cycling protocol comprises an initial activation at 95ºC for 12 min, followed by 40 cycles of denaturation at 95ºC for 15–20 s and annealing/extension at 60ºC for 60 s. Reaction setup can be performed at room temperature. Routine storage is at -18ºC to -28ºC, and shipping or temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product. The product is manufactured by Solis BioDyne in compliance with ISO 9001 and ISO 13485 certified quality management systems. It is supplied for research use only and is not for use in diagnostic procedures.