HOT FIREPol® Probe qPCR Mix Plus (Capillary)

Solis BioDyne's HOT FIREPol® Probe qPCR Mix Plus (Capillary) is a 5x concentrated master mix optimized for real-time quantitative PCR using DNA hydrolysis probes that rely on 5' flap endonuclease activity. The mix contains all components necessary to perform qPCR, with the exception of template, primers and probe, and is formulated for detection of low copy number targets. Mix composition includes HOT FIREPol® DNA Polymerase, a 5x Probe qPCR buffer providing 15 mM MgCl2 (3 mM at 1x), dNTPs, and BSA. The included polymerase is activated by a 12 minute incubation at 95°C, which prevents extension of non-specifically annealed primers and primer-dimers formed during reaction setup.

Recommended applications include detection and quantification of DNA and cDNA targets, gene expression profiling, microbial detection, and viral load determination. The suggested protocol comprises an initial activation step at 95°C for 12 minutes, followed by 40 cycles of denaturation at 95°C for 15–20 seconds and annealing/extension at 60°C for 60 seconds. The product is manufactured by Solis BioDyne under ISO 9001 and ISO 13485 certified Quality Management Systems and is supplied for research purposes only, not for use in diagnostic procedures. Routine storage is at -18°C to -28°C.