HOT FIREPol® Multiplex qPCR Mix (ROX)

A probe-based qPCR master mix from Solis BioDyne, optimized for amplifying up to 4 targets in a single reaction in real-time quantitative PCR assays. The HOT FIREPol® Multiplex qPCR Mix (ROX) is supplied at 5x concentration and contains HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, MgCl2, and ROX passive reference dye. It is designed for DNA hydrolysis probes based on 5' flap endonuclease activity. The polymerase is activated by a 10 minute incubation at 95°C, which prevents extension of non-specifically annealed primers and primer-dimers formed during reaction setup.

The 5x multiplex qPCR buffer provides 15 mM MgCl2, giving a final concentration of 3 mM MgCl2 in the 1x PCR solution. The mix includes dNTPs containing dUTP, allowing optional UNG treatment to prevent carryover contamination from previous runs; UNG is not included in the mix and must be purchased separately. The ROX passive reference dye is used to normalize the fluorescent reporter signal in qPCR and is compatible with both low-ROX and high-ROX system requirements. If ROX, Texas Red or a similar dye is used as one of the fluorophores, the passive reference might interfere with the signal; versions without passive reference or with Purple reference dye are also available.

Recommended applications include detection and quantification of DNA and cDNA targets, gene expression profiling, microbial detection, and viral load determination. Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product; routine storage is at -18°C to -28°C. The product is manufactured by Solis BioDyne under ISO 9001 and ISO 13485 certified Quality Management Systems and is supplied for research use only.