HepatiCult OGM Medium (Human)

From STEMCELL Technologies, HepatiCult™ Organoid Growth Medium (Human) is a cell culture medium designed to support the reliable generation, expansion, and differentiation of physiologically relevant human liver organoids under serum-free culture conditions.

Within the HepatiCult™ Organoid Kit (Human) workflow, the medium is recommended for the expansion phase following initiation in HepatiCult™ Organoid Initiation Medium (OIM; Human), typically starting from human liver tissue-derived hepatic ducts. Established organoids are maintained in HepatiCult™ OGM for 2-3 passages before being switched to HepatiCult™ Organoid Differentiation Medium (ODM; Human) to promote differentiation toward more mature hepatic cell types. Organoids expanded in this medium display characteristics of proliferating hepatic progenitors, expressing stem cell markers such as LGR5 and AXIN2, ductal markers SOX9 and KRT19, and the hepatic marker HNF4A, with minimal albumin expression observed during the expansion phase.

For Matrigel® dome cultures, domes are seeded with 5,000 - 15,000 live cells and cultures typically require passaging every 10 - 12 days. For scale-up in dilute Matrigel® suspension cultures, approximately 1,000 - 3,000 fragments per mL of OGM + Matrigel® is recommended, with fragment sizes targeted at 30 - 100 μm and passaging every 4 - 7 days. Once thawed, HepatiCult™ Organoid Growth Supplement should be used immediately or aliquoted and stored at -20 °C; aliquots must not be re-frozen. Complete OGM + Y-27632, if not used immediately, can be stored at 2 - 8 °C for up to 2 weeks. California Proposition 65 warning: this product can expose users to Progesterone, a chemical known to the State of California to cause cancer.