Gel and PCR Clean-up Kit, 250Preps

A membrane-based clean-up system from STEMCELL Technologies is used to extract DNA from agarose gels or to purify PCR products and restriction enzyme digests using a benchtop microcentrifuge. The kit purifies DNA fragments of 100 bp to 10 kb in length from agarose gel slices, or directly from the products of enzymatic reactions such as polymerase chain reaction (PCR) amplification or restriction digestion, efficiently removing enzymes, nucleotides, salts, and other impurities.

The purified DNA is suitable for downstream applications such as automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion, or in vitro transcription. Up to 40 µg of DNA can be processed per column in as little as 20 minutes and eluted in volumes as low as 15 µL, with up to 95% recovery depending on DNA fragment length. The system can be used to purify linear DNA fragments, supercoiled plasmid DNA, or single-stranded DNA (circular or linear).

The kit is based on the ability of DNA to bind to silica membranes in the presence of chaotropic salts and is supplied as a complete set of components not available for individual sale. STEMCELL Technologies' quality management system is certified to ISO 13485, and the product is intended for research use only.