Estradiol (E2)-6-HRP Antigen

Antibodies.com supplies this Estradiol (E2)-6-HRP Antigen as a liquid antigen-HRP conjugate in a 1 ml volume. The reagent functions as a labeled competitor in competitive ELISA workflows that quantify the steroid hormone estradiol. The estradiol moiety is covalently coupled at the C6 position to horseradish peroxidase, producing a stable reporter complex that yields a strong enzymatic signal upon binding by an anti-estradiol antibody. By incorporating this pre-conjugated reagent, researchers developing in vitro immunoassays can skip an independent HRP labeling step and proceed directly to assay optimization and screening.

In a typical working format, the conjugate is combined with test sample and a capture antibody, then developed with a chromogenic substrate. The resulting signal is inversely proportional to estradiol concentration in the sample, supporting sensitive competitive detection schemes. Because the conjugate is supplied as a ready-to-dilute liquid, it streamlines pipetting and minimizes preparation time at the bench. The 1 ml pack size accommodates numerous plate-scale runs or extended assay development projects. Refrigerate the vial and protect from light to preserve the catalytic activity of the HRP reporter over multiple freeze-thaw events. Intended for research use only.