ES-Cult M3120, 40mL

Mammalian cell culture laboratories maintaining mouse embryonic stem cells (mESCs) depend on purpose-formulated media to preserve pluripotency and minimize spontaneous differentiation. This 40 mL bottle of ES-Cult medium from STEMCELL Technologies delivers a ready-to-use, defined supplement system that supports self-renewal when combined with leukemia inhibitory factor (LIF) on gelatinized or feeder-free substrates. Researchers employ the medium across gene editing, transgenic line derivation, embryoid body formation, blastocyst injection, and in vitro differentiation workflows.

The optimized formulation reduces batch-to-batch variability and promotes consistent colony morphology. Each lot is quality tested for the maintenance of alkaline phosphatase activity and pluripotency marker expression in validated mESC lines. Equilibrate to 37 °C before use, protect from light, and store refrigerated when not in active service; avoid repeated freeze-thaw cycles to preserve cytokine and growth factor activity. The 40 mL single use format supports typical small-to-mid scale culture schedules and integrates with companion STEMCELL Technologies reagents, including ES-Cult-grade passaging and cryopreservation products, for end-to-end embryonic stem cell workflows.

The medium is intended for research use only and is not approved for diagnostic or therapeutic applications. Laboratories working on knockout mouse production, CRISPR-engineered line generation, or developmental biology studies will find it well matched to routine mESC maintenance demands.