ClonaCell-HY Medium D without HAT

A methylcellulose-based semi-solid cloning medium from STEMCELL Technologies, this formulation (ClonaCell-HY Medium D without HAT) supports the growth and clonal proliferation of hybridoma cell lines after the initial selection phase. Used in monoclonal antibody production workflows, the medium enables individual hybridoma colonies to be visualized, picked, and expanded for downstream antibody generation. The "without HAT" designation indicates that the supplement combination of Hypoxanthine, Aminopterin, and Thymidine has been omitted, making it appropriate for established hybridoma cultures that have already been selected, as well as for routine passaging and banking of stable antibody-producing clones.

Researchers commonly aliquot the medium into single-use working volumes and store it refrigerated to preserve its semi-solid gelling properties and protect colony-forming performance. The product is supplied in a bottle format and is intended for research use only, supporting cell culture applications such as hybridoma cloning, scale-up, and recombinant antibody line development. By providing a defined semi-solid environment, the medium enables discrete colony formation without the overlay steps required by soft agar alternatives, streamlining the workflow from hybridoma fusion to stable clone isolation.