ClonaCell-HY Liquid HAT Selection Medium

A 500 mL ready-to-use liquid hybridoma selection medium from STEMCELL Technologies combining hypoxanthine, aminopterin, and thymidine (HAT) in a serum-containing DMEM base for monoclonal antibody production workflows. The aminopterin blocks the de novo nucleotide biosynthesis pathway in unfused myeloma cells, while hypoxanthine and thymidine rescue hybridomas via the salvage pathway, so only successfully fused B-lymphocyte–myeloma cells survive. The formulation is supplemented with gentamicin, 2-mercaptoethanol, phenol red, L-glutamine, and pre-qualified serum, supporting outgrowth across mouse, rat, hamster, and human lymphocyte backgrounds. The medium is added directly to fused cell suspensions in culture vessels, applied 24 to 72 hours after PEG-mediated fusion or electrofusion, and supports the selection phase before downstream screening or methylcellulose-based cloning. Each bottle is functionally tested for hybridoma outgrowth performance and is supplied as a single-use research format for cell culture and in vitro monoclonal antibody generation only. Store frozen at -20°C, protect from light, and aliquot upon thawing; thaw at room temperature or overnight under refrigeration, avoiding a 37°C water bath. Not approved for diagnostic or therapeutic use.