6.5 mm Transwell Inserts, 48 Inserts

From STEMCELL Technologies, the Costar® 6.5 mm Transwell® inserts are permeable supports with 0.4 µm pore polyester membranes, designed to support the growth of both anchorage-dependent and anchorage-independent cells, including those that are fed at the basolateral surface or used in co-culture systems. Each insert conforms to the standard well dimension of a 24-well plate, with a clear and transparent polystyrene plate, lid, and transwell body, and a tissue culture-treated transparent polyethylene terephthalate (PET) membrane. The inserts are sterilized by gamma irradiation, are recommended for storage at 15–25°C, and remain stable until the expiry date on the label.

These inserts are recommended for culturing airway epithelial cells at the air-liquid interface and have been validated by STEMCELL Technologies for use with PneumaCult™-ALI Medium. In side-by-side comparisons with competitor inserts of the same material and pore size, cultures generated using these inserts showed superior ALI culture morphology and higher expression of goblet (MUC5B) and ciliated (FOXJ1) cell markers, producing a pseudostratified mucociliary epithelium that closely resembles the human airway in vivo. The inserts are noted for low lot-to-lot variability, supporting reproducible results when combined with the serum-free and BPE-free PneumaCult™-ALI formulation.

Typical applications include air-liquid interface culture, anchorage-dependent and anchorage-independent cell culture, basolateral feed cell culture, and co-culture systems. Products are for research use only and are not intended for human or animal diagnostic or therapeutic uses unless otherwise stated. STEMCELL Technologies Inc.'s quality management system is certified to ISO 13485.