3T3-J2 Irradiated Feeder Cells, 0.5 mL

Irradiated 3T3-J2 mouse fibroblasts from STEMCELL Technologies are cryopreserved feeder cells designed to support epithelial cell expansion in Conditional Reprogramming (CR) Medium. The cells are mitotically inactivated by radiation while retaining metabolic activity, so they can be used directly for seeding without prior plating.

Each vial contains 2 - 3 x 10^6 cells, sufficient to seed up to three T-25 cm² flasks or one T-75 cm² flask. When co-cultured with primary epithelial cells in complete CR Medium, a seeding density of 3 x 10^4 viable cells/cm² (range 2.5 - 4 x 10^4 viable cells/cm²) is recommended, with incubation at 37°C and 5% CO₂ and full medium changes every 2 - 3 days.

The product is stable at -135°C or colder for 12 months from the date of receipt. Short-term storage at -80°C (less than 1 month) is acceptable when minimized to maintain viability. Thawed samples must be used immediately. Storage in the vapor phase of a liquid nitrogen tank is recommended; liquid phase storage is discouraged due to contamination and explosion risks. A face shield is required when transferring cells, and sharps such as needles and syringes should not be used. For Research Use Only.