Z-VAD-FMK, 5 mg

STEMCELL Technologies offers a cell-permeable synthetic peptide that inhibits caspases and blocks caspase-mediated apoptosis in vivo. The compound prevents differentiation and enhances the freeze-thaw survival rate of human embryonic stem cells under cryopreservation conditions. Published applications include blocking Fas-induced apoptosis in T lymphocytes.

The product is supplied as a crystalline solid and is stable at -20°C as supplied, with protection from prolonged exposure to light recommended. For long-term storage, store with a desiccant, and the product is stable as supplied for 12 months from date of receipt.

The compound is soluble in DMSO at concentrations up to 10 mM, though it has low solubility in aqueous media. For use as a cell culture supplement, stock solution should be diluted into culture medium immediately before use, and final DMSO concentration should be kept below 0.1% to avoid potential cell toxicity. Alternative names include Z-Val-Ala-Asp-(OMe)-fluoromethyl ketone and ZVAD(OMe)-FMK, with CAS Number 187389-52-2, chemical formula C22H30FN3O7, molecular weight of 467.5 g/mol, and purity reported as ≥ 95%.