Soybean Trypsin Inhibitor, 1 g

Neutralize residual trypsin activity during cell dissociation and harvesting with this lyophilized Soybean Trypsin Inhibitor (SBTI) from STEMCELL Technologies. The 1 g vial delivers a ~20 kDa Kunitz-type serine protease inhibitor that binds trypsin with high affinity, forming a stable complex and protecting cell surface proteins from proteolytic cleavage.

Typical working concentrations fall between 0.1 and 1 mg/mL, allowing researchers to scale the inhibitor load to the trypsin dose in their protocol. SBTI is added directly to cell suspensions following enzymatic release from primary tissue or adherent monolayers, and it is equally useful during protein extraction workflows where unwanted proteolysis must be minimized. The reagent is compatible with downstream applications including flow cytometry, immunohistochemistry, and in vitro assays.

Store the lyophilized powder at -20 degrees C in the original sealed vial, protected from moisture, for long-term stability. Reconstitute in sterile buffer, aliquot, and freeze single-use portions to preserve activity across multiple harvests.

Provided as a research-use-only reagent, SBTI complements STEMCELL Technologies' broader portfolio of cell isolation media, dissociation enzymes, and culture supplements, helping standardize passaging and primary cell recovery workflows.