Y-27632 (2HCl), 5x10mg

From STEMCELL Technologies comes a small-molecule inhibitor of Rho-associated, coiled-coil containing protein kinase (ROCK), supplied as the dihydrochloride salt form of Y-27632. The product is featured in research workflows for iPS cell generation using a non-integrating, self-replicating RNA reprogramming vector. [S1]

The compound is reported as a selective ROCK1 and ROCK2 inhibitor, exhibiting greater than 200-fold selectivity over other kinases including PKC, cAMP-dependent protein kinase, MLCK and PAK, with reported Ki values of 140 nM for ROCK1 and 300 nM for ROCK2 in cell-free assays. [S2]

In published research with human embryonic stem (hES) cells, treatment at 10 μM was reported to markedly diminish dissociation-induced apoptosis, increase cloning efficiency from approximately 1% to 27%, and support SFEB-cultured hES cell survival and differentiation into Bf1+ cortical and basal telencephalic progenitors. [S2]