Y-27632 (2HCl), 500 mg

A selective small-molecule inhibitor of Rho-associated coiled-coil kinase (ROCK) supplied as the dihydrochloride salt, this 500 mg research-grade reagent from STEMCELL Technologies supports cell biology workflows including maintenance of human pluripotent stem cells, organoid culture, and cryopreservation recovery. Y-27632 inhibits both ROCK1 and ROCK2 by competing with ATP at the catalytic site, reducing downstream myosin light chain phosphorylation and cytoskeletal tension.

Researchers commonly add the compound to culture media during single-cell passaging of iPSCs and ESCs, during thawing of frozen cell stocks, and during transient transfection protocols where apoptosis limits yield. The inhibitor improves post-dissociation survival, supports colony formation from low-density seeding, and stabilizes three-dimensional epithelial organoid systems.

Supplied as a lyophilized powder in a single-use vial containing 500 mg. Reconstitute in sterile water or DMSO to prepare a 10 mM stock solution, aliquot, and freeze at -20 degrees C protected from light. The lyophilized powder remains stable when refrigerated; avoid repeated freeze-thaw cycles of working aliquots. For research use only; not intended for diagnostic or therapeutic applications.