Y-27632 (2HCl), 5 mg

Selective inhibition of Rho-associated coiled-coil kinase (ROCK) makes Y-27632 one of the most widely used small-molecule tools in stem cell biology and regenerative medicine research. Provided as the dihydrochloride salt in a 5 mg vial, this inhibitor is routinely applied to improve survival of dissociated human embryonic and induced pluripotent stem cells, enhance single-cell cloning efficiency in CRISPR editing workflows, and support organoid and intestinal stem cell culture systems.

The compound is supplied as a lyophilized powder and is typically reconstituted in water or DMSO to a 10 mM stock solution. For most hESC and iPSC passaging applications, the working concentration is 10 µM, though protocols vary by cell type. The powder should be stored at room temperature, protected from light, while reconstituted stock solutions should be aliquoted and stored at -20 °C to minimize freeze-thaw cycles.

STEMCELL Technologies manufactures this research-grade inhibitor for in vitro research use only. The product is commonly referenced in workflows involving cell dissociation, cryopreservation recovery, and three-dimensional organoid culture. Researchers should consult peer-reviewed protocols for species-specific and cell-type-specific dosing guidance.