Y-27632 (2HCl), 1 mg

A selective, cell-permeable inhibitor of Rho-associated coiled-coil kinase (ROCK) isoforms ROCK1 and ROCK2, Y-27632 dihydrochloride is widely adopted in stem cell and regenerative biology workflows. The dihydrochloride salt form improves aqueous solubility for direct addition to culture media without organic solvent pre-dilution. This 1 mg vial is routinely applied at working concentrations of 5 to 10 uM to enhance single-cell survival during standard passaging of human pluripotent stem cells (hESCs and iPSCs), to suppress anoikis in dissociated cell populations, and to support efficient organoid and 3D culture establishment.

Beyond hPSC maintenance, the compound is used in CRISPR-Cas9 genome editing workflows, somatic cell reprogramming, hematopoietic progenitor expansion, neural rosette formation, and corneal endothelial cell preservation. It is supplied as a lyophilized powder in a single use vial; reconstitute in sterile water to a 10 mM stock, aliquot into tubes, and store stocks frozen at -20 degrees C to limit freeze-thaw loss. Protect from light and avoid repeated freeze-thaw cycles; the dry powder remains stable at room temperature. The product is intended for in vitro research use only.

STEMCELL Technologies supplies this reagent as part of its cell culture portfolio, supporting reproducible PSC workflows, robust single-cell cloning, and downstream differentiation protocols.