Trichostatin A, 1 mg

Supplied as a 1 mg single-use vial of off-white lyophilized powder, this small-molecule epigenetic modifier from STEMCELL Technologies reversibly inhibits class I and class II histone deacetylases by chelating the catalytic zinc ion at the enzyme active site. Reported IC50 values for the compound include 6 nM against HDAC1, 8.6 nM against HDAC6, and 38 nM against HDAC4, placing it among the most potent broad-spectrum HDAC inhibitors used in cell biology research.

In pluripotent stem cell workflows, treating cultures with 50 nM TSA during reprogramming of mouse embryonic fibroblasts has been shown to increase induced pluripotent stem cell (iPSC) colony yield roughly three-fold, while 100 nM treatment boosts efficiency up to seven- to eight-fold. The same compound is reported to enhance CRISPR-Cas9 gene editing in human iPSCs at working concentrations near 6.25 ng/mL, raising on-target editing rates two- to four-fold without increasing off-target indels. Beyond reprogramming, TSA is routinely applied to chromatin remodeling studies, cancer cell cycle and apoptosis assays, hematopoietic and neuronal differentiation protocols, and investigations of acetylation-dependent transcriptional regulation.

The lyophilized powder dissolves readily in DMSO to prepare concentrated stock solutions. Store the vial at -20 °C, protected from light, and keep reconstituted aliquots refrigerated for short-term use or frozen for longer-term storage. Each vial is intended for research use only and is a single-use format intended for laboratory-scale epigenetic, stem cell, and oncology studies.